Fig. 2

IRF4 activates the transcription of TEX41 in melanoma cells. A Three TFs (IRF4, FOXM1 and FOXD3) both potentially binding to TEX41 and up-regulated in SKCM were displayed as Venn diagram and selected out by bioinformatics analysis via Human TFDB (Predicted score >= 15) and GEPIA (log2 fold change > 2 and p < 0.05). B Expression levels of three candidate TFs in melanoma cells were detected via qRT-PCR. C ChIP assay was conducted to measure the affinity between the candidate TFs and TEX41 promoter. D The efficiency of IRF4 overexpression and knockdown was assessed by qRT-PCR. E Schematic diagram of binding sites between IRF4 and TEX41 promoter was predicted by Human TFDB and JASPAR. F Luciferase reporter assays were implemented to verify the binding affinity between IRF4 and TEX41 promoter. G The expression of TEX41 was detected by qRT-PCR in A375 and SK-MEL-2 cells with IRF4 augment or depletion. ** P < 0.01