Fig. 1

Ivermectin inhibits proliferation of ESCC cells in vitro. A. Viability of cells was assessed by MTT assay. B. The morphological changes of ESCC cells were observed by phase contrast microscopy at 200× magnification, Scale bar = 50 μm; The anti-proliferative effect of ivermectin on ESCC cells was determined by LDH assay (C); colony formation (D), Scale bar = 10 mm; and EdU incorporation assays (E), Scale bar = 50 μm; F. Cell cycle of ESCC cells after treatment with was performed by flow cytometry. Values represent mean ± S.E.M. of 3 independent experiments. ^*P < 0.05, ^**P < 0.01; ^***P < 0.001.