Fig. 8

SGC-7901-secreted exosomal miR-107 reversed drug resistance of SGC-7901/5-FU cells by inhibiting HMGA2. The SGC-7901 cells were transfected with miRNA-107 inhibitor, co-transfection with miRNA-107 inhibitor and siRNA traget for HMGA2 and miRNA-107 inhibitor/siHMGA2 negative control for 24 h, and the cell viability of SGC-7901/5-FU cells after treated with different concentrations of 5-FU (a) or CDDP (b) combined with exosomes extracted from SGC-7901 (NC, 107 i, 107 i + siHMGA2) cells for 48 or 24 h, respectively. Cell viability was determined by MTT assay. Cells treated with vehicle serve as a blank control. Abbreviations: Exo, exosomes. All experiments were conducted in quintuplicates and data were expressed as the mean ± SD (n = 5). Statistical significances were determined using one-way ANOVA followed by Dunnett’s test. ^*P < 0.05, ^**P < 0.01, compared with negative control transfection group; ^#P < 0.05, ^##P < 0.01, compared with miRNA-107 inhibitor/siHMGA2 co-transfection group; ns, no significance (P > 0.05), compared with the negative control transfection and miRNA-107 inhibitor/siHMGA2 co-transfection groups