Fig. 8

OS curves based on macrophage properties and the density and distribution pattern of macrophage infiltration characterized by CD68⁺ and CD163⁺ immunoreactivity in the tumor nest (TN) and tumor stroma (TS). A–D Overall survival curves by CD68⁺ positive macrophage infiltration into the tumor nest (A) and stroma (C). Overall survival curves by CD163⁺ positive M2 macrophage infiltration into the tumor nest (B) and stroma (D). Representative images of high density of CD68⁺ staining (E) and CD163⁺ staining (F) in TN and TS. Representative images of low density of CD68⁺ staining (G) and CD163 staining (H) in TN and TS. Scale bar, 100 μm