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Table 3 Concentrations and supplier details of reagents used for flow cytometry

From: The effect of hypoxia on PD-L1 expression in bladder cancer

Name

Details

Dilution

Supplier

Code

Live/dead stain

Diluted in PBS

1:1000

Invitrogen

L34973

Fc blocking

Diluted in FACs buffer

1:100

Miltenyi Biotec

130–059-901

PD-L1 antibody

PE conjugated

1:100

Fisher Scientific

12–5983-41

IgG antibody

PE conjugated

1:100

BD Pharmigen

554,121