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Table 2 Primer sequences and their properties used for qPCR

From: The effect of hypoxia on PD-L1 expression in bladder cancer

Primer

Sequence

Tm (°C)

GC-content (%)

PD-L1 forward

TATGGTGGTGCCGACTACAA

57.3

50

PD-L1 reverse

TGGCTCCCAGAATTACCAAG

57.3

50

SDHA forward

CATCCACTACATGACGGAGCA

59.8

52.4

SDHA reverse

ATCTTGCCATCTTCAGTTCTGCTA

59.3

41.7