Table 1 Concentrations and supplier information of antibodies used for Western blotting
From: The effect of hypoxia on PD-L1 expression in bladder cancer
Target | Raised in | Dilution | Supplier | Code |
|---|---|---|---|---|
PD-L1 | Rabbit | 1:2000 | Cell Signalling Technology | 13,684 |
HIF1α | Mouse | 1:500 | BD | 610,959 |
GAPDH | Rabbit | 1:2500 | Cell Signalling Technology | 2118 |
Mouse-HRP | Goat | 1:7500 | Invitrogen | 62–6520 |
Rabbit-HRP | Goat | 1:5000 | Invitrogen | 65–6120 |