Fig. 5

In silico analyses show a positive association between hypoxia and IFNγ-signalling in muscle-invasive bladder tumours. Hypoxia scores were applied to tumours from BCON and TCGA cohorts filtered to include stage 2 and above only. A published 6-gene IFNγ gene signature was used to attribute an IFNγ score to each tumour. These scores were plotted against the hypoxia scores in the A) BCON and C) TCGA cohorts. R² values were calculated using Pearson’s correlation coefficient and the p values represent a linear model analysis. Tumours were stratified into hypoxia low or high using the median of the hypoxia score from each cohort, and plotted against the IFNγ signature score in the B) BCON and D) TCGA cohorts. P values were calculated using one way ANOVA between the two groups. p values are represented as follows: ns = not significant, * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001