Fig. 2
From: The effect of hypoxia on PD-L1 expression in bladder cancer
Hypoxia (0.1 and 1%) decreases PD-L1 expression in a panel of human bladder cancer cells. Flow cytometry analyses show the surface expression of PD-L1 decreases after culture in hypoxia across a panel of human bladder cancer cells. Culture in 0.1 and 1% O2 for 24 h decreases the expression of PD-L1 compared with the levels present when cultured under 20% O2 in T24 (A), J82 (B), UMUC3 (C) bladder cancer cells. Cells were seeded and left to adhere for 24 h before placing in 0.1% or 1% O2 for 24 h and 10 ng/mL IFNγ added to the relevant wells. Data are the mean ± standard error of the mean (SEM) of the mean fluorescence intensity of 10,000 viable cells from replicates of at least two independent experiments normalised to the normoxia untreated condition to show the relative fold change. Statistical tests are unpaired t tests performed in GraphPad Prism with p values represented as follows: ns = not significant, * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001