Fig. 5
From: MMP12 knockout prevents weight and muscle loss in tumor-bearing mice
MMP12 Degrades Insulin and Insulin-like Growth Factor-1. A Representative images of the peak were detected at 488 nm after the insulin peptide was degraded by normal mice serum. The insulin peptide was labeled with FAM (488 nm) and DABCLY (quenching fluorescence). When the insulin peptide was broken, FAM was then observed at 488 nm (resonance energy transfer, FRET). B The synthetic insulin (or IGF-1) peptide was labeled with FAM and DABCLY as shown. The insulin peptide was labeled with FAM (488 nm) and DABCLY (quenching fluorescence). When the insulin (IGF-1) peptide was broken, FAM was then observed at 488 nm. Fluorescence intensity of the peak were detected at 488 nm after the insulin (IGF-1) peptide was degraded by MMP12 using a fluorescence microplate reader. Electrospray Ionization Mass Spectrometry (IMS) was used to detect its characteristic peaks. C The MMP12 and insulin (or IGF-1) peptide interaction led to appearance of a fluorescence signal on dose-dependent (***P < 0.001, **P < 0.01; data are shown as means ± SD)