Fig. 7

Hsa_circ_0043278 regulates EI24 and NF-κB expression by sponging miR-455-3p. A qRT–PCR and B western blotting were used to examine the relative expression of EI24 in MDA-MB-231 cells after transfection of the hsa_circ_0043278 overexpression vector and the miR-455-3p mimic as well as in MCF-7 cells after transfection of hsa_circ_0043278 shRNA and the miR-455-3p inhibitor. (The images of the western blot bands were cropped from the images shown in Figs. S9 and S10). C Relative protein levels of NF-κB (P65) in the cytosol or nucleus of MDA-MB-231 and MCF-7 cells in each group were determined by western blot analysis. (The images of the western blot bands were cropped from the images shown in Figs. S11, S12, S13, and S14.) The data are presented as the mean ± standard deviation values (n = 3); * P < 0.05, *** P < 0.001, **** P < 0.0001