Fig. 3

Hsa_circ_0043278 inhibited the tumorigenesis of BC cells in vivo. A Images of xenograft tumours from BALB/c nude mice in each group after injection of MDA-MB-231 cells with overexpression of hsa_circ_0043278 or MCF-7 cells with downregulation of hsa_circ_0043278. B The tumour weights were measured in each group. C Tumour growth in each group was monitored weekly after injection of transfected cells. D The protein levels of NF-κB (P65) in tumours were examined by immunohistochemical staining, and the corresponding quantification of nuclear P65 was performed. Scale bar, 50 μm. The data are presented as the mean ± standard deviation values (n = 3); * P < 0.05, ** P < 0.01, **** P < 0.0001. IOD, integrated optical density