Fig. 2

Hsa_circ_0043278 decreased BC cell viability and inhibited their migration and invasion. A Schematic illustration of the overexpression vector and siRNA of hsa_circ_0043278. The expression of (B) hsa_circ_0043278 and (C) TADA2A was verified by qRT–PCR in MDA-MB-231 and MCF-7 cells after upregulation or downregulation of hsa_circ_0043278, respectively. D The growth of MDA-MB-231 and MCF-7 cells in each group was analysed by MTT assays. E The viability of MDA-MB-231 and MCF-7 cells in each group was evaluated by colony formation assays. F The migration capacities of MDA-MB-231 and MCF-7 cells in each group were examined by wound healing assays. Scale bar, 200 μm. G The migration and (H) invasion abilities of MDA-MB-231 and MCF-7 cells in each group were analysed by Transwell assays. Scale bar, 100 μm. The data are presented as the mean ± standard deviation values (n = 3); * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001