Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: X-ray repair cross-complementing protein 1 (XRCC1) loss promotes β-lapachone –induced apoptosis in pancreatic cancer cells

Fig. 2

β-lap exposure results in significant DNA base damage. A. Distribution patterns of XRCC1 in untreated MiaPaCa2 cells and cells treated with 6 μM of β-lap for 30 min or 60 min. B. Relative mRNA expression levels of XRCC1 from PAAD tumor (n = 179) and associated normal pancreas (n = 171) samples. C&D. Relative survival of MiaPaCa2 shXRCC1 and BxPC3 shXRCC1 cells treated with β-lap for 2 h. E. Immunostaining of γH2AX (red) in shScr and shXRCC1 MiaPaCa2 cells was monitored after 1 h treatment of 3 μM β-lap with or without dicoumarol (50 μmol/L, 2 h). Nuclei were counterstained with DAPI. bar 10 μm. F. MiaPaCa2 cells were treated with 3 μM β-lap for 1 h with or without dicoumarol (50 μmol/L, 2 h) before performing Comet Assay (alkaline electrophoresis conditions, 23 V for 30 min). Cells were exposed to 2 mM H2O2 for 20 min (in PBS) as positive control. Comet tail moment were measured by CASP software. Shown are representative images of experiments performed at least three times. Shown are comet tail means ±SEM of at least 50 comet tails per condition. *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05. G. Relative H2O2 levels in MiaPaCa2 cells treated with or without various β-lap doses (μmol/L, 2 h), ± dicoumarol (50 μmol/L, 2 h). Values are mean ± S.D. n = 3. *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05 compared with shScr controls. ### p ≤ 0.001; ## p ≤ 0.01; #p ≤ 0.05 compared with shXRCC1 controls

Back to article page