Table 1 Primers and sequences
From: The genotypic and phenotypic impact of hypoxia microenvironment on glioblastoma cell lines
Name | Forward primer (5′- 3′) | Reverse Primer (5′- 3′) |
|---|---|---|
miR- 34-5p | ACACTCCAGCTGGGTGGCAGTGTCTTAGCT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGACAACC |
miR-128-3p | ACACTCCAGCTGGGTCACAGTGAACCGGTC | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGAAAGAGAC |
miR-221-3p | ACACTCCAGCTGGGAGCTACATTGTCTGCT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGGAAACCCA |
miR- 17-5p | ACACTCCAGCTGGGCAAAGTGCTTACAGTG | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGCTACCTGC |
miR-181a-3p | ACACTCCAGCTGGGACCATCGACCGUUGAT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGGGTACAAT |
miR-181b-5p | ACACTCCAGCTGGG- AACATTCATTGCTGTC | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGACCCACCG |
miR-181c-5p | ACACTCCAGCTGGGAACATTCAACCTGTCG | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGACTCACCG |
Universal reverse | Â | CTCAAGTGTCGTGGAGTCGGCAA |
SNU6 | GCTTCGGCAGCACATATACTAAAAT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGCGTTCCA |
Human Actin | ATGAAGATCAAG ATCATTGCTCCT | ACATCTGCTGGAAGGTGGACA |