Fig. 9
From: The genotypic and phenotypic impact of hypoxia microenvironment on glioblastoma cell lines
Dysregulation of miRNAs in GBMs under hypoxia by RT-qPCR. The GBM cell lines were cultured under hypoxic conditions. RT-qPCR was run using the SYBR green platform. The relative fold change difference was calculated using 2-ΔΔCq method where RNU6 was used as the normalizer. The graphs were drawn and analysed using t test by graph pad prism. Each value represents the mean ± SD of three independent runs and * indicates p < 0.05; compared to the astrocytes grown in either normoxia or hypoxia conditions