Fig. 8
From: The genotypic and phenotypic impact of hypoxia microenvironment on glioblastoma cell lines
Increased expression of an astrocytic and a cytoskeleton protein hypoxia. A & C. Cells were cultured under normoxia (N) and another set in hypoxia (H) for 72 h and stained for GFAP) and vimentin by immunocytochemistry. The Images were taken with DMi8 Leica microscope and prepared and quantified using ImageJ. B & D. Western blotting was done by the SDS-PAGE method and densitometry done using ImageJ. The relative expression was normalized to tubulin. The graphs were drawn and analysed using t test by graph pad prism. Each value represents the mean ± SD of three independent experiments, * indicates p < 0.05. Scale bar = 50 μm. Representative Western blots are shown as cropped images. Full-length blots are presented in Additional Files 6 & 7 (Fig. S6 and S7)