Fig. 6
From: The genotypic and phenotypic impact of hypoxia microenvironment on glioblastoma cell lines
Enhanced expression of VEGF under hypoxia. A. Cells were cultured under normoxia (N) and another set in hypoxia (H) for 72 h and stained for VEGF by immunocytochemistry. The Images were taken with DMi8 Leica microscope and quantified using ImageJ. B. Western blotting was done by the SDS-PAGE method and densitometry done using ImageJ. The relative expression was normalized to tubulin. The graphs were drawn and analysed using t test by graph pad prism. Each value represents the mean ± SD of three independent experiments, * indicates p < 0.05. Scale bar = 50 μm. Representative Western blots are shown as cropped images. Full-length blots are presented in Additional File 4 (Fig. S4)