Skip to main content
Fig. 5 | BMC Cancer

Fig. 5

From: Cellular retinol binding protein-1 inhibits cancer stemness via upregulating WIF1 to suppress Wnt/β-catenin pathway in hepatocellular carcinoma

Fig. 5

CRBP-1 regulates WIF1 expression through indirectly activating its promoter transcription. (A) The intracellular RA analysis in CRBP-1 overexpressed PLC/PRF/5 cells and control cells. All data shown represent the mean ± SD from three independent experiments. (B) qPCR analysis of the expression of representative RAR/RXR target genes including RARB2, CYP26A1, and HOXa1 in CRBP-1 overexpressed PLC/PRF/5 cells and control cells. Data shown represent the mean ± SD from three independent experiments. (C) Western-blot analysis of the expression of WIF1 in PLC/PRF/5 and Huh7 cells after treated with/without 5 μM RA for 48 h. (D) Western-blot analysis of the expression of WIF1 in CRBP-1 overexpressed PLC/PRF/5 and Huh7 cells as well as control cells after treated with/without 5 μM RA for 48 h. (E) Cell proliferation analysis of CRBP-1 overexpressed PLC/PRF/5 and Huh7 cells as well as control cells after treated with/without 5 μM RA for 48 h. Data shown represent the mean ± SD from three independent experiments. (F) The prediction analysis of RAREs in the promoter region of WIF1. (G) The dual-luciferase activity analysis for WIF1 promoter in 293 T cells in response to CRBP-1 overexpression. Data shown represent the mean ± SD from three independent experiments. (H) ChIP analysis of RAREs for WIF1 promoter in CRBP-1 overexpressed PLC/PRF/5 cells

Back to article page