Fig. 7

METTL3/YTHDF2 m6A axis regulates the expression of miR-1915-3p by suppressing KLF4. A qRT-PCR analysis of miR-1915-3p expression in A549 and H1975 cells transfected with METTL3 siRNA or YTHDF2 siRNA. B The relative expression of pri-miR-1915 and pre-miR-1915 were checked by qRT-PCR after YTHDF2 inhibition. C KLF4 expression were enhanced following METTL3 or YTHDF2 knockdown in lung cancer cells. D miR-1915-3p expression was analyzed in A549 and H1975 cells transfected with KLF4 siRNA or KLF4 expression vector. E Schematic diagram showing the KLF4-putative binding locus in the vicinity of the TSS. F Promoter activity of miR-1915 in A549 cells was examined using dual luciferase assays. G KLF4 increased the transcription activity of miR-1915 promoter, and deleting both KLF4-binding sites diminished KLF4-mediated reporter induction. H CHIP assays were conducted using anti-KLF4 or anti-IgG antibody. Precipitated DNA from A549 and H1975 cells was analyzed by PCR. Normal IgG and Ab-(antibody-free) were served as negative control groups, and 1% cell lysates was used as input control