Fig. 5

RNA editing differs between total RNA compared to mRNA. (A) A method to calculate junction-corrected RNA editing level. To strictly calculate the RNA editing levels using mRNA reads, we only kept reads for which the read or its mated pair were mapped to splice junctions. The “spliced reads” were then used to calculate the mRNA editing levels. (B) Difference between overall RNA editing level and junction corrected editing level. Top, the histogram of mean editing level change. Bottom, volcano plot illustrating the difference and p-values for each editing site, comparing overall to (junction-corrected) mRNA editing levels. (C) Examples of editing sites showing significant editing level change comparing overall RNA editing level to mRNA editing level. The overall and mRNA editing level from the same sample are connected with black lines to indicate paired connections