Skip to main content
Fig. 4 | BMC Cancer

Fig. 4

From: TGF-β protects osteosarcoma cells from chemotherapeutic cytotoxicity in a SDH/HIF1α dependent manner

Fig. 4

Metabolic succinate facilitated osteosarcoma chemo-resistance through an HIF1α dependent manner. A The expression of HIF1α in Saos-2 and MG-63 cells after treatment with TGF-β (50 ng/ml) or succinate (20 mM) was determined by western blot. B The expression of HIF1α in shSTAT1- or shSDHD-Saos-2/MG-63 cells was determined by western blot. C Saos-2 and MG-63 cells were treated with MTX (75 mM)/CIS (40 μM) plus KC7F2 (20 μM). Cell apoptosis was determined by flow cytometry. D Saos-2 and MG-63 cells were treated with MTX (75 mM) in the presence of TGF-β (50 ng/ml) plus KC7F2 (20 μM). Cell apoptosis was determined by flow cytometry. E The same as D except that Saos-2 and MG-63 cells were treated with CIS (40 μM) in the presence of TGF-β (50 ng/ml) plus KC7F2 (20 μM). F Tumorigenicity of Saos-2 and MG-63 cells after treatment with TGF-β (20 μg/kg) plus KC7F2 (10 mg/kg). SUC, succinate; MTX, methotrexate; CIS, cisplatin

Back to article page