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Fig. 3 | BMC Cancer

Fig. 3

From: Decreased sphingomyelin (t34:1) is a candidate predictor for lung squamous cell carcinoma recurrence after radical surgery: a case-control study

Fig. 3

H&E images (magnification 200×) (A, F), IHC images (magnification 200×) of p40 (B, G) and p63 (C, H), and mass spectra of [SM(t34:1) + H]+ (ID: 1526) (D, I) and [PC (12:0_12:0) + H]+ (E, J) were compared between representative recurrent and non-recurrent cases. Lipid profiles of the two cases are from recurrent case 4 and non-recurrent case 6 (Additional file 2, sheet 1). The H&E and IHC images for both the recurrent and non-recurrent cases show moderately differentiated SQCC with diffuse positive staining of p40 and p63 demonstrating no obviously apparent differences between the two cases, whereas mass spectrum intensities of [SM(t34:1) + H]+ (ID: 1526) are markedly reduced in the recurrent case. The high mass resolution obtained in this study can be viewed as a monoisotopic peak and two isotopic peaks of [SM(t34:1) + H]+ (ID: 1526). Monoisotopic peaks for [SM(t34:1) + H]+ (ID: 1526) were normalized to the monoisotopic peaks for [PC (12:0_12:0) + H]+. Abbreviations: H&E, Hematoxylin-eosin; ID, identity number; IHC, immunohistochemistry; PC, phosphatidylcholine; SM, sphingomyelin; SQCC, squamous cell carcinoma

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