Fig. 4

USP3 regulates Aurora A protein stability, thereby modulating Aurora A protein levels. A Aurora A levels were measured after ECa109 cells were knocked down of USP3. B USP3 was overexpressed while knocking down USP3, and Aurora A levels were detected. C-D After knocking down USP3, the protein degradation promoter CHX was added to detect Aurora A levels. E-F. After overexpressing USP3, the protein degradation promoter CHX was added to detect Aurora A levels. G-H. After knocking down and overexpressing USP3, the protein degradation inhibitor MG132 was added to detect Aurora A levels