Fig. 5
From: NOX4-derived ROS-induced overexpression of FOXM1 regulates aerobic glycolysis in glioblastoma
FOXM1 is critical for NOX4-induced aerobic glycolysis and proliferation in glioblastoma cells. A Western blot analysis showing the protein expression of the indicated genes in U87MG cells. B U87MG cells were infected with NOX4 overexpression (NOX4) plasmid and FOXM1 knockdown (shFOXM1) lentiviral vectors before plating in a Seahorse XFe96 analyzer and assessment with a glycolysis stress test. C-D Overexpression of NOX4 promoted the production of lactate and cellular ATP, while knockdown of FOXM1 reversed these effects, in U87MG cells. E-F CCK-8 and EdU assays showing the proliferation of the indicated U87MG cells stably expressing NOX4 + shFOXM1. *, P < 0.05; **, P < 0.01; ***, P < 0.001