Fig. 8

BTG2 is direct target of miR-93-5p. (A) Schematic representation of the miR-93-5p predicted binding sites in the 3′-UTRs of BTG2 mRNAs and 3′-UTR-mutated alignments. (B) Dual-luciferase reporter assay. The luciferase activities of the mutated vectors of BTG2 were unaffected by the transfection of miR-93-5p.(C) Western blotting assay. MiR-93-5p significantly inhibited the expression of BTG2. (D),(E). qRT-PCR analysis. MiR-93-5p significantly inhibited expression level of BTG2 mRNA in T24 and UM-UC-3 cells. The relative levels were presented as the fold change referred to corresponding NC. *** represents p<0.001, **** represents p < 0.0001, ns represents not-significant