Fig. 2

FAM83H-AS1 directly targets miR-485-5p in HCC cells. (A) The location of FAM83H-AS1 in HCC cells was detected. (B-C) 6 candidate miRNAs were predicted to bind to FAM83H-AS1 through the combined analysis of lncRNASNP2 database (http://bioinfo.life.hust.edu.cn/lncRNASNP#!/) and DIANA tools (http://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=lncbasev2/index-predicted). (D) The expression of these candidate miRNAs in HCC cells and THLE-3 cells was evaluated via RT-qPCR analysis. (E) Detection of miR-485-5p expression was performed via RT-qPCR analysis in FAM83H-AS1-depleted HCC cells. (F) The potential binding site between FAM83H-AS1 and miR-485-5p predicted by lncRNASNP2 was shown. (G) RT-qPCR was applied to validate the upregulation efficiency of miR-485-5p. (H) The luciferase activity of FAM83H-AS1-WT/Mut was detected in miR-485-5p-overexpressed HCC cells. (I) The enrichment of FAM83H-AS1 or miR-485-5p in Anti-Ago2 bound precipitates was detected in RIP assays. ^*P < 0.05, ^**P < 0.01