Fig. 9
Dnmt3a negatively regulates miR-17-5p and miR-17-5p represses Cdkn1a expression in Jak2V617F BaF3 cells. A. The expression level of miR-17-5p in Jak2V617F BaF3 cells and control BaF3 cells. B. The verification of the knockdown and overexpression effect of Dnmt3a in Jak2V617F BaF3 cells. Full-length blot images are presented in Supplementary Fig.19. C. The expression level of miR-17-5p in Jak2V617F BaF3 cells with Dnmt3a knockdown and overexpression. D. The verification of the effect of transfection of miR-17-5p mimic and inhibitor in Jak2V617F BaF3 cells. E & F. The transcription and expression of Cdkn1a in Jak2V617F BaF3 cells transfected with miR-17-5p mimic or inhibitor. Full-length blot images are presented in Supplementary Fig.19. G. Graphic abstract of the present study