Fig. 2
Dnmt3a expression is repressed by Stat5a and Stat5a is the upstream regulator of Dnmt3a. A. The expression levels of p-STAT5a and DNMT3a proteins in human myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. 5. B. The expression levels of p-Stat5a and Dnmt3a proteins in murine myeloid tumor cell lines. Full-length blot images are presented in Supplementary Fig. 6. C. In wild type BaF3 cells, the protein expression level of Dnmt3a after Stat5a was knocked down. Full-length blot images are presented in Supplementary Fig. 7. D. In wild type BaF3 cells, the protein expression level of Dnmt3a after overexpression of Stat5a. Full-length blot images are presented in Supplementary Fig. 8. E & F. In Jak2V617F BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. 9 and Supplementary Fig. 10. G & H. In wild-type BaF3 cells, the protein expression of Stat5a after knockdown or overexpression of Dnmt3a. Full-length blot images are presented in Supplementary Fig. 11 and Supplementary Fig. 12