Fig. 5

Effect of GLRX3 knockdown in cancer stemness-related molecules in pancreatic cancer cells and the mRNA expression in TCGA data: A After shRNA-mediated GLRX3 knockdown in HPAC cells, GLRX3-silenced cell lines (G10 and H10) presented altered expression of EMT-related proteins. E-cadherin and beta-catenin were upregulated and Wnt family, RhoA, RhoB and ABCG2 presented altered expression compared to the control cells; B-C showed the representative results of well-correlated TCGA data analyses with GLRX3 K/D cell line studies. B GLRX3 and stemness related gene expression levels in pancreatic cancer tissues were downloaded from the TCGA by using ISB Cancer Genomics Cloud. Outlier samples were removed by clipping the top and bottom deciles of each expression distribution. The correlation between GLRX3 and representative gene mRNA expression is illustrated as a scatter plot where each dot represents a single cancer tissue sample. The Pearson’s correlation value (R), and the p value are indicated; C GLRX3 and stemness related gene mRNA expression and cancer patients’ clinical data were derived from the TCGA database. Patients were divided into low (red line) or high (blue line) expression groups, using maximally selected rank statistics. Survival of patients was visualized using Kaplan–Meier plots. The log-rank p values between the groups are shown in each plot