Fig. 4
From: LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKA
LINC00958 sponged miR-490-3p in bladder cancer cells. A TargetScan showed the predicted binding sequences of miR-490-3p for LINC00958. B Dual luciferase assay was performed in cells co-transfected with plasmids LINC00958-Wt or LINC00958-Mut and NC mimics or miR-490-3p mimic in RT4 and T24 cells. C RT-qPCR detection of LINC00958 expression on enrichment of miR-490-3p by RIP assay. D RT-qPCR detection of miR-490-3p expression in bladder cancer tissues (n = 34) and normal tissues (n = 34). E Correlation analysis between miR-490-3p expression and LINC00958 expression. F RT-qPCR detection of miR-490-3p expression in SV-HUC-1, RT4, and T24 cell lines. G RT-qPCR detection of LINC00958 and miR-490-3p expression in RT4 and T24 cells transfected with NC, Si-lnc, inhibitor, and Si-lnc + inhibitor. *, P < 0.05; **, P < 0.001 compared to blank. #, P < 0.05; ##, P < 0.001 compared to Si-lnc + inhibitor. Blank, blank control; NC, negative control; Wt, wild-type; Mut, Mutant; Si-lnc, siRNA-LINC00958; inhibitor, miR-490-3p inhibitor