Fig. 5

Comparison of surface protein expression in EVs from Her2 positive and Her2 negative patients show that CD29 and CD146 are increased in Her2 positive patients. A Background corrected CD9/CD63/CD81 normalized intensity signal for the surface proteins CD29 and CD146 that differed between Her2+ and Her2- patients in the MACSPlex analysis. (B-C) CD146 was further evaluated by western blot (10 μg protein was loaded in each lane except for patient 2 where 9 μg was used due to low protein concentration). B Western blot and C the normalized western blot quantification for CD146. Western blot membranes in the figure were cropped, uncropped membranes and the unstained gel used for the normalization are shown in Additional file 7. The Imaging and data analysis was done in Image Lab™ Software (Bio-Rad Laboratories). CD146 was normalized and quantified by Stain-Free Total Protein Quantitation and the normalized volume intensity values are shown in the figure. Results shown as mean values and standard deviation. **p < 0.01, q < 0.01