Fig. 2

BZM enhanced MTX-induced anti-proliferation activity. A-D. CFSE-stained LNCaP or 22RV1 cells were treated with vehicle, MTX(1 μM), BZM (100 nM) or combination (MTX, 0.5 μM; BZM (50 nM). CFSE decay was determined by flow cytometry. A, C. Plots of flow cytometry. B, D. Quantitative data of CFSE decay. E. Cells proliferation was determined by CCK-8. Quantitative data were deduced from triplicate experiments and presented as means ± s.e.m. The asterisks indicate significant differences (one-way ANOVA, **p < 0.01, ***p < 0.001)