Fig. 1
BZM enhanced MTX anti-tumor activity in vivo. A-C, Subcutaneous xenografts were established with LNCaP cells in male SCID mice. Two weeks after injection, mice were treated with BZM (1 mg/kg, intraperitoneally, twice weekly), MTX (3 mg/kg, intraperitoneally, every day), or combination (0.5 mg/kg BZM, twice weekly; 1.5 mg/kg MTX, every day) (n = 10, per group). Tumor growth and animal survival rate were monitored. A. Quantitative data for tumor growth curve. Tumor sizes were measured at the indicated days with a caliper and calculated as [length x width2]/2(n = 10, per group). Data are presented as means ± s.e.m. The asterisks indicate significant differences (two-way ANOVA, **p < 0.01). B. Tumor growth curves of individual mice (n = 10, per group). C. Overall animal survival. The asterisks indicate significant differences (n = 10, per group). (log-rank test, *p < 0.05, **p < 0.01)