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Table 2 Fluorescence in situ hybridization (FISH) on sorted blasts

From: Blast cells surviving acute myeloid leukemia induction therapy are in cycle with a signature of FOXM1 activity

Biobank number Cytogenetic details FISH pre-chemotherapy FISH post-chemotherapy Probe used
64 46, XY, inv.(3)(q21q26), del(7)(q22) [10] Failed Insufficient material NA
121 45, XX, inv.(3)(q21q26), −7[8]/46, XX [2] 44/45 1G1O 1/45 1G 83/120 1G1O 37/120 1G Vysis D7S522/CEP7
205 44, XX, add(3)(p25), −5, −7[12] 61/100 1G1O 39/100 1G Insufficient material Vysis D7S522/CEP7
285 Normal    
349 Normal    
494 45 ~ 49, XY, −4, −5, −7, del(9)(q?22),? der(15;17) (q10;q10), + 21, del(22)(q13), + 2 ~ 5mar[cp10] Insufficient material 92/100 1G1O
8/100 1G
Vysis D7S522/CEP7
  1. Where sufficient cells were available, FISH was used to confirm clonality of sorted blast populations. Samples 285 and 349 had normal cytogenetics and no target for FISH. Samples 121, 205 and 494 had monosomy 7 which was detected using two probes: CEP7 (green probe targeting the centromere, 7p11.1-q11.1) and D7S522 (orange probe targeting 7q31). 1G1O, 1 green and 1 orange signal per cell; 1G, 1 green signal per cell