Table 2 Probe and primer sequences for NPY and WIF1 genes
Gene | Oligo type | Sequence | Fluorophore | Tm (°C) |
|---|---|---|---|---|
NPY | Forward primer | 5′ CGCGGCGAGGAAGTTTTATA 3′ | / | 58 |
Reverse primer | 5′ ATACTATCGAACGAACGTCTCCG 3′ | / | 64 | |
Probe | 5′ CGCGATTCGTTTTTTGTA 3’ | Cyanine 5 | 47.8 | |
WIF1 | Forward primer | 5′ GAGGGAGTTGTAGCGTAGTAGAGTATTTG 3’ | / | 58 |
Reverse primer | 5′ AAAACTCCTCGTACCGCACCTA 3’ | / | 54 | |
Probe | 5′ CGGCGTTAGGTTGC 3’ | Cyanine 3 | 56 |