Fig. 4

Microcell development and GFP expression after 24 h doxorubicin (DOX) treatment in SK-MEL-28 cells. The images were taken every 2 h; the red squares mark the region of interest (ROI). At 0, 2, 4, 6, 8, and 10 h, the cell was rounded, looking morphologically similar to the beginning of apoptosis (white arrow); at 12, 14, 16, and 18 h, the start of development of microcells (16–18 h, white arrow) and the expression of EGFP could be observed. At 20 h (white arrow), microcells were fully developed and expressed GFP. The scale bar is 100 μm