Fig. 2

Peritoneal fluid (PF) stimulates cancer cell proliferation. A. Mia PaCa-2, Panc1, and Aspc1 cell lines were incubated for 4 days in the presence of PF at the indicated concentrations. As a positive control, foetal bovine serum (FBS) was used at the same concentration as PF. Incubated cells were treated with WST-1 solution, and absorbance was detected at 450 nm. B. Cells were incubated in the presence of PF until the colonies were formed. Colonies were stained with crystal violet, and the percentage of colony areas were calculated using ImageJ software. Columns correspond to the mean of three independent experiments; bars indicate SD. Statistical analyses were performed using the unpaired t-test