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Fig. 3 | BMC Cancer

Fig. 3

From: CircCDC45 promotes the malignant progression of glioblastoma by modulating the miR-485-5p/CSF-1 axis

Fig. 3

MiR-485-5p was one of the targets of circCDC45. (A) The miRNAs targeted by circCDC45 was predicted by the online tools starBase and circbank. (B and C) The expression of predicted miRNAs in LN229 and A172 cells with circCDC45 overexpression was detected by qRT-PCR. (D) The wild and mutant circCDC45 sequence fragments were amplified and cloned into luciferase reporter plasmid. (E and F) The relationship between circCDC45 and miR-485-5p was validated by dual-luciferase reporter assay. (G and H) The relationship between circCDC45 and miR-485-5p was validated by RIP assay. (I) The expression of miR-485-5p in LN229 and A172 cells with circCDC45 knockdown was detected by qRT-PCR. (J and K) The expression of miR-485-5p in clinical tissues and cell lines was also detected by qRT-PCR. (L) Spearman’s correlation analysis revealed the correlation between miR-485-5p expression and circCDC45 expression in GBM tissues. *P < 0.05

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