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Fig. 4 | BMC Cancer

Fig. 4

From: Co-expression of YAP and TAZ associates with chromosomal instability in human cholangiocarcinoma

Fig. 4

YAP/TAZ control proliferation- and CIN-associated genes in CCA cells. A List of identified KEGG pathways and sub-categories with lowest NES after combined YAP/TAZ inhibition in HUCCT-1 cells (FDR ≤ 0.05). B Heatmap summarizing significantly regulated genes of ‘cell cycle’ (hsa04110) after combined YAP/TAZ inhibition in HUCCT-1 cells (FDR ≤ 0.05). Genes not significantly regulated are excluded. Genes that were used for independent qPCR confirmation are indicated (*). C Independent confirmatory qPCR experiment in NOZ cells with genes identified in the KEGG pathways cell cycle (hsa04110; CDK2, PLK1), DNA replication (hsa03030; MCM3, MCM7) and Hippo signaling pathway (hsa04392; AJUBA, TEAD4). D Heatmap summarizing qPCR results from 16 genes that are part of the CIN25 signature in HUCCT-1 cells. CYR61 and ANKRD1 served as positive controls. Two YAP- and TAZ-specific siRNAs were transfected individually and in combination. E Exemplary bar graphs for the CIN signature genes CCNB1, CCNB2, TOP2A and TTK in HUCCT-1 cells after YAP/TAZ inhibition

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