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Fig. 3 | BMC Cancer

Fig. 3

From: Co-expression of YAP and TAZ associates with chromosomal instability in human cholangiocarcinoma

Fig. 3

YAP/TAZ transcriptionally regulate and support cholangiocyte-derived cancer cell proliferation. A Real time PCR for YAP and TAZ in HUCCT-1 and NOZ cells after combined siRNA-mediated silencing of YAP and TAZ for 24 h. B Western immunoblotting and detection of YAP, TAZ and CYR61 in total protein lysates (HUCCT-1 and NOZ) 24 h after transfection of YAP/TAZ-specific siRNAs. GAPDH served as loading control. C Heatmaps summarize the results of real-time PCRs after combined YAP/TAZ silencing in HUCCT-1 and NOZ cells for 24 h. Known YAP/TAZ target genes were analyzed. D HUCCT-1 and NOZ cell viability assays were measured 48 and 72 h after transient transfection of YAP/TAZ-specific siRNA combinations. E Cell proliferation of HUCCT-1 and NOZ cells was measured 48 h after combined YAP/TAZ silencing. Scr. - scrambled siRNA (control). Statistical test: Mann-Whitney U

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