Fig. 2

YAP and TAZ shuttle dynamically in cholangiocyte-derived cancer cells. A Detection of YAP and TAZ in total protein lysates from different cholangiocyte-derived cancer cell lines. iCCA cells (HuH-28, SNU1079, HUCCT-1), eCCA cells (SNU478) and gallbladder carcinoma cells (G415, NOZ, GB-d1) were tested. Actin served as loading control. HUCCT-1 (B) and NOZ (C) cells were cultured under variable cell density conditions. Exemplary immunofluorescence images with YAP and TAZ in cells for low- and high-density conditions are shown. Graphs illustrate the nuclear/cytoplasmic (n/c) ratio for YAP (red) and TAZ (blue). Higher n/c ratios indicate nuclear enrichment, while low ratios represent nuclear exclusion. Each dot represents one image. The dashed line defines the mean cell count over all fields of vision of the cell line. Values smaller than this threshold define low cell density and values above this threshold indicate high cell density. Violin plots summarize the results of all obtained images. Statistical test: Mann-Whitney U. Lines represent linear regression for YAP (red) and TAZ (blue)