Fig. 7

The tumor-specific pattern of BATF2 expression in serum and exosome in BC cases. (A) Morphology of serum-sourced exosomes under TEM. The red arrows indicate isolated vesicles. (B) Detection of exosomal marker proteins as CD9 and CD63 in isolated vesicles by immunoblotting. Cytochrome C (mitochondrial marker) was used as a negative control to exclude the possible mixture of cellular contamination. (C, D) The expression level of BATF2 mRNA in BC serum and the (E) plotted ROC curve used to identify BC. (F, G) Decreased expression of serum-derived exosomal BATF2 in BC patients, and (H) the ROC curve of exosomal BATF2 in confirming BC. ***P < 0.0001