Fig. 4
From: Synergistic efficacy of inhibiting MYCN and mTOR signaling against neuroblastoma
Combined effects of JQ1 and TEM on neurosphere formation. (a) Representative micrograph of spheres of SK-N-BE2 cells with the treatment of control-(DMSO) or JQ1 (0.5 μM) and TEM (2 μM) alone or combined for 7 days. Scale bar, 1000 μm. (b) Quantification of the number of neurospheres following treatments. The values represent mean ± SEM. **p < 0.01; ***p < 0.001 (Student-t-test). (c) Western blot analysis for the expression of neural stem cell markers following treatment of neurospheres with JQ1 (0.5 μM) and TEM (2 μM) alone or combined for three days. Cyclophilin was used as the loading control in this analysis. The original uncropped images of these blots are provided in the Additional File 1 (Fig. S3)