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Fig. 2 | BMC Cancer

Fig. 2

From: Synergistic efficacy of inhibiting MYCN and mTOR signaling against neuroblastoma

Fig. 2

Combined effects of JQ1 and TEM on cell cycle arrest and apoptosis. (a) Representative flow cytometry plots show cell cycle distribution in SK-N-BE2 cells treated with JQ1 (0.5 μM) and TEM (2 μM) alone or combined for 24 h. On the right, graphs show the quantification of cell cycle distribution in two MYCN-amplified (SK-N-BE2, SK-N-DZ) NB cell lines treated with JQ1 (0.5 μM) and TEM (2 μM) alone or combined for 24 h. (b) Representative flow scatter diagrams show apoptosis induction in SK-N-BE2 cells treated with JQ1 (0.5 μM) and TEM (2 μM) alone or combined for 72 h. On the right, bar graphs show the quantification of Annexin-V/PI double positive apoptotic cells in two MYCN-amplified (SK-N-BE2, SK-N-DZ) and one non-MYCN-amplified (SK-N-AS) NB cell lines treated with JQ1 (0.5 μM) and TEM (2 μM) alone or combined for 72 h. Values, mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 (Student-t-test)

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