Fig. 5

The target relationship between miR-216a-5p and BCL2L2. (A) The binding site of miR-216a-5p and BCL2L2. (B) Luciferase activity was examined in CAL27 cells with transfection of control vector, WT-BCL2L2–3’UTR or MUT-BCL2L2–3’UTR and miRNA NC or miR-216a-5p mimic. (C) BCL2L2 expression was examined in OSCC and normal samples. Sample size: n = 30. (D) BCL2L2 level was detected in OSCC cells (CAL27 and SCC25) and HOK cells. (E) miR-216a-5p abundance was examined in CAL27 and SCC25 cells with transfection of miRNA NC or miR-216a-5p mimic. (F) BCL2L2 level was detected in CAL27 and SCC25 cells with transfection of miRNA NC, miR-216a-5p mimic, miR-216a-5p mimic + pc-NC or pc-BCL2L2. (G) BCL2L2 abundance was examined in CAL27 and SCC25 cells with transfection of si-NC, si-circ_0011946#1, si-circ_0011946#1 + inhibitor NC or miR-216a-5p inhibitor. ^*P < 0.05