Fig. 3

The target relationship between circ_0011946 and miR-216a-5p. (A) The binding sequence of circ_0011946 and miR-216a-5p. (B) Luciferase activity was measured in CAL27 cells with transfection of control vector, WT-circ_0011946 or MUT-circ_0011946 and miRNA NC or miR-216a-5p mimic. (C and D) circ_0011946 and miR-216a-5p levels were measured after RNA pull-down using oligo or circ_0011946 probe. (E) miR-216a-5p level was determined in OSCC and normal samples. Sample size: n = 30. (F) miR-216a-5p abundance was examined in OSCC cells (CAL27 and SCC25) and HOK cells. (G) miR-216a-5p level was examined in CAL27 and SCC25 cells with transfection of inhibitor NC or miR-216a-5p inhibitor. (H) miR-216a-5p abundance was detected in CAL27 and SCC25 cells with transfection of si-NC, si-circ_0011946#1, si-circ_0011946#1 + inhibitor NC or miR-216a-5p inhibitor. ^*P < 0.05