Skip to main content
Fig. 6 | BMC Cancer

Fig. 6

From: Uev1A promotes breast cancer cell migration by up-regulating CT45A expression via the AKT pathway

Fig. 6

Effects of CT45A and UEV1A expression on EMT signaling in breast cancer cells. a, b Relative transcript levels of EMT markers, including epithelial markers E-cadherin and mesenchymal markers N-cadherin, vimentin in CT45A-overexpressed MDA-MB-231 (a) and MCF7 (b) cells were determined by qRT-PCR. c The expression of N-cadherin and E-cadherin in CT45A-overexpressed MDA-MB-231 (left panel) and MCF7 (right panel) cells was detected by western blot against anti-N-cadherin and anti-E-cadherin antibodies. d The expression of N-cadherin and E-cadherin in MDA-MB-231 (left panel) and MCF7 (right panel) cells transiently overexpressing UEV1A and depleted of CT45A was detected by western blot using anti-N-cadherin and anti-E-cadherin antibodies. The gel images in (c) and (d) are cropped from available original blots. Numbers underneath the WB images indicate relative band intensity after normalization with the loading control. e-h Statistical analyses of relative cellular N-cadherin and E-cadherin levels in MDA-MB-231 (e, g) and MCF7 (f, h) cells as indicated in the graphs. CK, control treatment; siNC, control siRNA. All experiments were performed in at least triplicate and the results are the average with standard deviation. **, P < 0.01; and ***, P < 0.001

Back to article page