Fig. 5

CT45A promotes cell migration of HCT116 colorectal cancer cells. a Cellular HA-tagged Uev1A and Uev1A-F38E were detected by western blot against an anti-HA antibody. b Overexpressed UEV1A but not UEV1A-F38E upregulated CT45A expression in HCT116 cells. c HCT116 cells were transfected with shRNA lentiviral particles against UEV1A (shUEV1A) or non-specific target (shCK). CT45A transcript levels in shCK and shUEV1A cell lines were monitored by qRT-PCR. d Cellular HA-tagged CT45A was detected by western blot against an anti-HA antibody. e Representative images of HCT116 cell migration using the transwell assay. f Statistical analysis of the cell migration assay data. Cells that migrated to the lower surface of the filter were counted in five random fields under a light-microscope at 100 × magnification. g Representative images of HCT116 cell migration ability using the transwell assay. HCT116 cells expressing UEV1A were depleted of CT45A and subjected to the transwell assay. h Statistical analysis of the cell migration assay data. CK, control treatment; siNC, control siRNA. All experiments were performed in at least triplicate and the results are the average with standard deviation. **, P < 0.01; and ***, P < 0.001. The gel images in (a) and (d) are cropped from available original blots