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Fig. 3 | BMC Cancer

Fig. 3

From: Uev1A promotes breast cancer cell migration by up-regulating CT45A expression via the AKT pathway

Fig. 3

Effects of altered UEV1A and CT45A expression on MDA-MB-231 cell migration. a The ectopic CT45A expression was monitored by western blot against an HA-tag antibody. The gel images are cropped from available original blots. b Representative images of cell migration using the transwell assay. c Statistical analysis of the cell migration assay data. Cells migrated to the lower surface of the filter were counted in five random fields under a light-microscope at 100 × magnification. d, e The relative expression of CT45A (d) and UEV1A (e) in CT45A-depleted and UEV1A transiently overexpressed cells was monitored by qRT-PCR. f Representative images of cell migration ability using the transwell assay. MDA-MB-231 cells transiently expressing UEV1A were depleted of CT45A and subjected to the transwell assay. g Statistical analysis of the cell migration assay data. Cells that migrated to the lower surface of the filter were counted in five random fields under a light-microscope at 100 × magnification. CK, control treatment; siNC, control siRNA. All experiments were performed in at least triplicate and the results are the average with standard deviation. **, P < 0.01; and ***, P < 0.001

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